Anti-GFAP antibody [EPR1034Y]

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Anti-GFAP antibody [EPR1034Y]

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货号：ab68428

品牌：abcam/艾博抗

计量单位：支

交货周期

：7

选择规格： 20uL 1mL 100uL 40uL

运杂费：登录后可查看运杂费

服务电话(移)：17319309550

DeepBio得分:5567.2

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产品详情

产品属性

退换货说明：不支持退换货	性状：固体	存储条件： ﹣20°
保质期： 1年

产品名称

Anti-GFAP抗体[EPR1034Y]
参阅全部 GFAP 一抗
描述

兔单克隆抗体[EPR1034Y] to GFAP - N-terminal
宿主

Rabbit
经测试应用

适用于: WB, IP, IHC-P, ICC/IF, mIHC, Flow Cyt (Intra), IHC-Frmore details
种属反应性

与反应: Mouse, Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- mIHC: Human cerebrum and cerebellum, mouse and rat cerebrum, mouse cerebellum, mouse hippocampus, mouse and rat spinal cord tissues.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.21% BSA
Concentration information loading...
纯度

Protein A purified
克隆

单克隆
克隆编号

EPR1034Y
同种型

IgG
研究领域
- Neuroscience
- Development

Alternative Versions
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human GFAP protein (ab114149)
- Recombinant Human GFAP protein (ab151370)
Related Products
- Human GFAP Matched Antibody Pair Kit (ab222279)

The Abpromise guarantee

Abpromise™承诺保证使用ab68428于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
WB		1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). For unpurified use at 1/50 000 - 1/100 000.
IP		1/20 - 1/40.
IHC-P	(9)	1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF	(2)	Use a concentration of 0.1 - 1 µg/ml.
mIHC		1/500 - 1/2000.
Flow Cyt (Intra)		1/500.
IHC-Fr	(3)	1/250.

说明
WB 1/10000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). For unpurified use at 1/50 000 - 1/100 000.
IP 1/20 - 1/40.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
ICC/IF Use a concentration of 0.1 - 1 µg/ml.
mIHC 1/500 - 1/2000.
Flow Cyt (Intra) 1/500.
IHC-Fr 1/250.

功能

GFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
组织特异性

Expressed in cells lacking fibronectin.
疾病相关

Defects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
序列相似性

Belongs to the intermediate filament family.
翻译后修饰

Phosphorylated by PKN1.
细胞定位

Cytoplasm. Associated with intermediate filaments.
Target information above from: UniProt accession P14136 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Entrez Gene: 24387 Rat
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- SwissProt: P47819 Rat
- Unigene: 514227 Human
- Unigene: 1239 Mouse
- Unigene: 91512 Rat
see all
别名
- wu:fb34h11 antibody
- ALXDRD antibody
- cb345 antibody
- etID36982.3 antibody
- FLJ42474 antibody
- FLJ45472 antibody
- GFAP antibody
- GFAP_HUMAN antibody
- gfapl antibody
- Glial fibrillary acidic protein antibody
- Intermediate filament protein antibody
- wu:fk42c12 antibody
- xx:af506734 antibody
- zgc:110485 antibody
see all

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] (ab68428)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat spinal cord tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat spinal cord.
Panel B: anti-GPR17 staining oligodendrocytes in rat spinal cord.
Panel C: anti-P2RY12 staining microglia in rat spinal cord.
Panel D: anti-GFAP staining astrocytes in rat spinal cord.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab218309, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] (ab68428)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse spinal cord tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse spinal cord.
Panel B: anti-GPR17 staining oligodendrocytes in mouse spinal cord.
Panel C: anti-P2RY12 staining microglia in mouse spinal cord.
Panel D: anti-GFAP staining astrocytes in mouse spinal cord.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab218309, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] (ab68428)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse hippocampus tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse hippocampus.
Panel B: anti-GPR17 staining oligodendrocytes in mouse hippocampus.
Panel C: anti-P2RY12 staining microglia in mouse hippocampus.
Panel D: anti-GFAP staining astrocytes in mouse hippocampus.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab218309, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] (ab68428)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebellum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebellum.
Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebellum.
Panel C: anti-P2RY12 staining microglia in mouse cerebellum.
Panel D: anti-GFAP staining astrocytes in mouse cerebellum.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/40000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab218309, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] (ab68428)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded rat cerebrum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on rat cerebrum.
Panel B: anti-GPR17 staining oligodendrocytes in rat cerebrum.
Panel C: anti-P2RY12 staining microglia in rat cerebrum.
Panel D: anti-GFAP staining astrocytes in rat cerebrum.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/140000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using ab218309, the same antibody clone in a different buffer formulation.
Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] (ab68428)

Fluorescence multiplex immunohistochemical analysis of formalin/PFA-fixed paraffin-embedded mouse cerebrum tissue sections.

Panel A: merged staining of anti-GPR17 (magenta; Opal™690), anti-P2RY12 (green; Opal™520) and anti-GFAP (red; Opal™570) on mouse cerebrum.
Panel B: anti-GPR17 staining oligodendrocytes in mouse cerebrum.
Panel C: anti-P2RY12 staining microglia in mouse cerebrum.
Panel D: anti-GFAP staining astrocytes in mouse cerebrum.

The section was incubated in three rounds of staining: in the order of ab316105 at a 1/500 dilution, ab300140 at a 1/140000 dilution, and ab218309 at a 1/1000 dilution for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system. Nuclear counterstaining with DAPI.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins.

The immunostaining was performed on a Leica Biosystems BOND^® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

This data was developed using