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Non-radioactive Rap1 Activation Assay Kit that uses Ral GDS RBD, agarose (Catalog # 14-455) to precipitate Rap1-GTP from cell lysates & detection by a Rap1 specific antibody.
NA.84
activity assay: suitable;affinity binding assay: suitable
human ... RAP1GDS1(5910)
P52306
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Kit capacity: 20-30 assays
Routinely evaluated by affinity precipitation assay. Ral GDS RBD, agarose precipitated GTP-Rap1 from HEK293 lysates. The precipitated GTP-Rap1 was detected by immunoblot analysis using Anti-Rap1.
100X GTPγS, 10mM (Cat.# 20-176) 100X GDP, 100mM (Cat.# 20-177) Rap1 Activation Lysis Buffer, 2X Anti-Rap1 Rap1 Assay Reagent (Ral GDS-RBD, agarose) (Cat.# 14-455)
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