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for DNA electrophoresis
NA.25
Lambda DNA Mixed Digest has been used for DNA electrophoresis.
For optimal resolution, the recommended agarose gel concentration is 0.4%.
Sigma’s Lambda DNA Mixed Digest contains 10 fragments from 1,503 − 48,502 bp. Six ul of the ladder should be diluted in gel loading buffer and then loaded in a single lane on an agarose or polyacrylamide gel.
Bacteriophage lambda DNA was completely digested with by Apa I, Kpn I, Xba I and Xho I in separate reactions, sticky ends filled with DNA polymerase I Klenow (to prevent reannealing), mixed with uncut bacteriophage lambda DNA, and then purified by phenol extraction.
10mM Tris-HCl 中的溶液，pH 8.0，1mM EDTA
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