产品简称

SYBR® Green qPCR reagent, passive reference dye included

NACRES

NA.55

抗体应用

qPCR: suitable

应用

SYBR® Green JumpStart™ Taq ReadyMix™ for High Throughput qPCR has been used for the amplification and quantification of transcripts to analyze gene expression in 2-step quantitative reverse transcription PCR (RT-qPCR). It has also been used for quantitative polymerase chain reaction (qPCR) of reverse-transcribed cDNA.

其他说明

SYBRGreen ReadyMix for High Throughput Quantitative PCR combines the performance enhancements of JumpStart Taq and SYBR Green I in an easy-to-use ReadyMix solution that incorporates ROX dye for ABI and other real time instrument applications. The ReadyMix includes a detection fluor, internal standard and reagents for PCR making it the ideal solution for performing high-throughput quantitative PCR.

一般描述

SYBR® Green JumpStart™ Taq ReadyMix™ is a ready-to-use 2X master mix that contains SYBR® Green I, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides (dNTPs), and reaction buffer making it the ideal solution for performing high-throughput quantitative PCR. SYBR® Green I dye binds to and detects any dsDNA generated during amplification. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.

包装

Default reaction volume is 50 μL 20RXN is packaged as 1 X 500 μL 400RXN is packaged as 1 X 10 mL 2000RXN is packaged as 1 X 50 mL

特点和优势

•The master mix allows consistency and reproducibility from one reaction to the next •Reduced set-up time as compared to manual or wax Hot Start methods •Reduced primer dimers •Allows for room temperature set-up; Ideal for high throughput, quantitative PCR application

法律信息

本品的使用受以下一个或多个美国专利和美国之外相应专利权利要求的保护：5,079,352、5,789,224、5,618,711、6,127,155、5,677,152（仅权利要求 1 至 23 项）、5,773,258（仅权利要求 1 和 6 项）、5,407,800、5,322,770、5,310,652、5,994,056、6,171,785，以及受对应于美国专利4,889,818 的美国之外的权利要求的保护。受前述专利权利要求的保护，本品的购买包括有限的、不可转让的豁免权，只可将该数量的产品用于购买者自己的内部研究。未通过暗示、默许明确让与任何其他专利权利要求（例如美国专利6,814,934 中的设备或系统权利要求）下的权利，以及进行任何商业服务的权利，包括但不限于出于有偿或其他商业考虑而报道购买者的实验结果。本品只可用于研究目的。受 Roche 专利保护的诊断用途需要获得 Roche 的单独许可。关于购买许可的更多信息，请联系许可总监 (Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA)。