NACRES

NA.56

应用

CelLytic Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris, and is immediately ready for affinity purification. CelLytic Express′ unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

一般描述

CelLytic™ Express is a non-denaturing and highly efficient protein extraction formulation for in-culture bacterial cell lysis. This proprietary powder formulation extracts 2-3 times more protein than conventional methods such as sonication. In addition, the method is fast and requires less sample manipulations, reducing proteolytic degradation and preserving protein activity. Unlike other in-culture lysis products, CelLytic™ Express is a complete formulation including lysozyme and endonuclease. The resulting lysate is completely clear of cellular debris and is immediately ready for affinity purification. CelLytic™ Express unique powder formulation adds minimal volume to the final lysate. It provides a cell lysis method with defined volume and time parameters, making it easier to validate and more convenient for production scale protocols.

特点和优势

•Efficient and non-denaturing in-culture protein extraction •Save time by eliminating cell harvest and clarification steps •Preserve biological activity with fewer manipulations •Compatible with affinity resins and magnetic beads •Ideal for Production Scale Extraction protocols

制备说明

CelLytic® Express is supplied ready-to-use. However, additional reagents may be used depending on the nature of the protein to be isolated. These include salts, protease inhibitors, and reducing agents such as dithiothreitol or 2-mercaptoethanol. The additional reagents should be added into the bacterial culture after dissolving the CelLytic™ Express, and not by adding them to the dry powder.

储存及稳定性

This product ships on wet ice and storage at –20°C is recommended. CelLytic™ Express, as supplied, is stable for at least two years when stored properly.