产品简称

pH 8.5-9.5 (25 °C), suitable for cell culture

NACRES

NA.75

抗体应用

cell culture | mammalian: suitable;single cell analysis: suitable

应用

Percoll®已用于以下材料的离心分离：新生大鼠心室心肌细胞血液样本中的白细胞小管周围肌样（PM）细胞适用于制备单细胞悬浮液以进行测序。

一般描述

Percoll® is a classic medium for density gradient centrifugation of cells, viruses, and subcellular particles. It consists of colloidal silica particles of 15-30 nm diameter (23% w/w in water), which have been coated with polyvinylpyrrolidone (PVP). The PVP coating renders the product completely non-toxic and ideal for use with biological materials. Due to its heterogeneity in particle size, sedimentation occurs at different rates, spontaneously creating very smooth, isometric gradients in the range of 1.0-1.3 g/ml.

特点和优势

•Low osmolality permits precise adjustment to physiological conditions without significant interference from the medium. •Compatible with living cells and viruses and allows separation and recovery of intact, fully active systems. •Impermeable to biological membranes, resulting in no change of buoyant density of particles during centrifugation. •Spontaneous formation of gradient during centrifugation allows mixing of large sample volumes in the centrifuge tubes. •Low viscosity results in rapid formation of gradients and particle separation. •Most biological particles having sedimentation coefficient values greater than 60S can be successfully isolated in Percoll gradients.