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With ʟ-glutamine and sodium bicarbonate, without HEPES, liquid, sterile-filtered, suitable for cell culture
NA.75
cell culture | mammalian: suitable
Dulbecco′s改良Eagle培养基/Ham F-12营养混合物已用于： •维持人肺和结直肠癌细胞系生存，进行次级代谢产物的生物学评估 •培养人肾-2细胞(HK-2)并作为检测培养基组分，用于溶血磷脂酰胆碱(LPC)处理 •小鼠鳞状细胞癌(SCCVII)肿瘤细胞培养
Media are supplemented with a defined mixture of nutrients, growth factors, and hormones instead of serum. A 1:1 mixture of Dulbecco′s Modified Eagle′s medium (DME) and Ham′s F-12 nutrient mixture is commonly used for these defined studies. To compensate for the loss of buffering capacity caused by eliminating serum, a final concentration of 15 mM HEPES buffer can be added to the formulations. It allows the investigator to optimize the buffering system.
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