产品简称

suitable for proximity ligation assay

产品线

Duolink®

生物来源

goat serum (polyclonal)

抗体应用

proximity ligation assay: suitable

应用

Application Note To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes. Other necessary reagents include a pair of PLA probes from different species (one PLUS and one MINUS), detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.HRP is also available for brightfield detection. Isotype control PLA probes are used in lieu of a primary antibody in a Duolink® PLA in situ experiment to determine background signal contributed by non-specific binding of the primary antibody. Isotype control PLA probes can also be used to determine the optimal titer conditions of primary antibody PLA probes. A MINUS probe of a different species must be used simultaneously with this product. See our Product Selection Guide for more information. Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects View full Duolink® product list

一般描述

An immunoglobulin G (IgG) isotype control antibody with the Duolink® PLA® PLUS oligonucleotide specific for goat IgG. This is a convenience product to be used in place of a goat primary antibody to determine non-specific background signal and optimal antibody dilution. The anti-goat PLA® probe PLUS is not necessary when using this product, but must be used in combination with a MINUS PLA® probe.

特点和优势

•No overexpression or genetic manipulation required •High specificity (fewer false positives) •Single molecule sensitivity due to rolling circle amplification •Relative quantification possible •No special equipment needed •Quicker and simpler than FRET •Increased accuracy compared to co-IP •Publication-ready results