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Widely used broad-spectrum protease mixture
water: 10 mg/mL
Pronase® 蛋白酶, 来源于灰色链霉菌,无核酸酶,已用于: •用于心脏侧群细胞(CSP)分析的单细胞制备 •黄曲霉毒素血浆生物标志物测定 •使秀丽隐杆线虫胚胎渗透以用于 体内 微球菌核酸酶消化
注意:1 KU = 1000 单位。
PRONASE® Protease, Streptomyces griseus, nuclease-free is a non-specific protease that liquefies mucins and digests proteins to free amino acids. This mixture can be used to isolate living chondrocytes. It is most active at pH 7.0 but stable over wide ranges of pH and temperature. It has an optimal pH of 7.5. Pronase is less cytotoxic and produces more efficient dissociation than collagenase.
Proteases catalyze the hydrolysis of proteins to form peptides and free amino acids. They also regulate the breakdown of fats and carbohydrates through many enzymatic cascades and activating proenzyme zymogens.
溶液中不稳定;仅在使用前进行复溶。
一个单位的定义为在40°C、pH 7.5下每分钟释放25 µg酪氨酸当量消化产物所需的酶量。
毒性:有害(C)
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