产品简称

affinity isolated antibody, buffered aqueous solution

NACRES

NA.46

抗体产品类型

secondary antibodies

生物来源

goat

单抗克隆号

polyclonal

偶连

peroxidase conjugate

抗体应用

direct ELISA: 1:35,000;dot blot: 1:40,000 (chemiluminescent);immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

应用

Anti-Human Lambda Light Chains (Bound and Free)?Peroxidase antibody produced in goat has been used in: •enzyme linked immunosorbent assay (ELISA) •immunohistology •dot blot •detecting and quantitating human lambda light chains (bound and free) via chromogenic, chemoluminescent or fluorogenic immunochemical or immunohistochemical techniques

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

一般描述

Mammalian antibodies contain one of two types of light chain, called kappa or lamba. Each chain contains a constant and a variable domain. Mammalian antibodies contain either two kappa or two lambda light chains. Horseradish Peroxidase (HRP) is an enzyme that catalyzes the conversion of chromogenic substrates such as o-phenylenediamine (OPD), 4-chloro-1-naphthol 3,3’,5,5’-tetramethylbenzidine (TMB), 3,3′-Diaminobenzidine (DAB) or 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS); chemiluminescent substrates such as CPS-3 (enhanced luminal) and fluorogenic substrates such as Ampliflu™ Red into detectable chromophores, light-emitters or fluorescers, respectively.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT.

制备说明

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).