产品简称

clone CTD4H8, from mouse, ALEXA FLUOR™ 488

NACRES

NA.41

抗体产品类型

primary antibodies

物种反应性

human, mouse

生物来源

mouse

单抗克隆号

CTD4H8, monoclonal

抗体分形

IgG1

偶连

ALEXA FLUOR™ 488

抗体应用

immunocytochemistry: suitable

免疫原基因信息

human ... POLR2A(5430)mouse ... Polr2A(20020)rat ... Polr2A(363633)

免疫原蛋白记录号

P24928

外形

Protein G Purified

应用

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected RNA polymerase II in HeLa cells.The unconjugated antibody (Cat. No. 05-623 & 05-623-Z) is shown to be suitable also for Western blotting, chromatin IP, ChIP-seq, and immunocytochemistry.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

其他说明

Concentration: Please refer to lot specific datasheet.

一般描述

DNA-directed RNA polymerase II subunit RPB1 (EC 2.7.7.6; EC 2.7.7.48; UniProt P24928; also known as DNA-directed RNA polymerase II subunit A, DNA-directed RNA polymerase II subunit RPB1, DNA-directed RNA polymerase III largest subunit, hRPB220, hsRPB1, RNA polymerase II subunit B1, RPBh1, RpIILS) is encoded by the POLR2A (also known as POLR2, POLRA, RPB1, RPO2, RPOL2) gene (Gene ID 5430) in human. RNA polymerase II (Pol II) is a 550 kDa, multi-subunit enzyme responsible for the transcription of protein-coding genes. Transcription initiation requires recruitment of the complete transcription machinery to a promoter via solicitation by activators and chromatin remodeling factors. Pol II can coordinate 10 to 14 subunits. This complex interacts with the promoter regions of genes and a variety of elements and transcription factors. The DNA binding domain of the polymerase is a groove where TFIIB orients the DNA for unwinding and transcription.

外形

Protein G Purified

质量

Evaluated by Immunocytochemistry in A431 cells.Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected RNA polymerase II in A431 cells.

目标描述

~210-220 kDa observed