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产品属性
退换货说明 : 质量问题可退化 |
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. In vitro, PDGF-BB stimulated MAP kinase activity and cell motility of isolated lymphatic endothelial cells. In vivo, PDGF-BB potently induced growth of lymphatic vessels. Expression of PDGF-BB in murine fibrosarcoma cells induced tumor lymphangiogenesis, leading to enhanced metastasis in lymph nodes. The PDGF signaling system contributes to and vascular remodeling. Growth factors such as PDGF exert potent effects on wound healing including the regeneration of tooth-supporting structures.
性质名称
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性质详情
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产品别称
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Becaplermin,ODGF,PDGF-2,PDGFBB,PDGF-BB
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种属
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Human
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表达序列
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Ser82-Thr190
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Accession
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P01127
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标签
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No Tag
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表达系统
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E.coli
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分子量
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Approximately 24.8 kDa
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纯度
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> 97% as determined by SDS-PAGE and HPLC.
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内毒素
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< 1.0 EU per μg by the LAL method.
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制剂
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Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4.
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外观
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Lyophilized Powder
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生物活性
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Fully biologically active when compared to standard. The ED50as determined by a cell proliferation assay using murine Balb/c 3T3 cells is less than 3 ng/ml, corresponding to a specific activity of > 3.3 × 10^5 IU/mg.
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复溶方法
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开盖之前,先离心,以将内容物带到底部。使用无菌蒸馏水或PBS复溶,重新配制至0.1-1.0 mg/mL的浓度,可根据后续实验更进一步稀释;长期储存,建议稀释缓冲液中加入载体蛋白(0.1% BSA);分装为一次实验用量,-80℃冻存,避免反复冻融。
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高纯度、高活性、低内毒素、高批间一致性
-25 ~ -15℃保存,收到货之后有效期1年。
复溶后,无菌条件下,2~8℃保存,7天有效期。复溶后, 无菌条件下,-85 ~ -65℃保存,3个月有效期。
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