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Rapid purification of PCR products for downstream procedures
The SpinPrep PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions. Column binding capacity: up to 6 g PCR volume: 100 l/rxn Typical recovery: 6090% Size range: 100 bp to > 12,000 bp Time required:< 10 min
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
•82 mlSpinPrep Bind Buffer •27 mlSpinPrep Wash Buffer •10 mlSpinPrep Elute Buffer •100SpinPrep Filters •100Receiver Tubes •100Eluate Receiver Tubes
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