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实验试剂 > 试剂盒 > 质粒DNA提取纯化
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70976-3,SpinPrep PCR Clean-up Kit

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货号:70976-3
品牌:Merck millipore/默克密理博
计量单位:
交货周期  
30天
选择规格: 100REACTIONS

运杂费:登录后可查看运杂费

所属公司:西格玛奥德里奇(上海)贸易有限公司

所 在 地:冼村路5号凯华国际中心1201-1204房

服务电话(移):18000000000

DeepBio得分:5567.2
DeepBio得分 是基于文献引用次数,影响因子,文献新近度等因素计算的客观产品评级,得分越高表明该产品经过越可靠的实验验证,质量可信度越高

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产品简称


Rapid purification of PCR products for downstream procedures



一般描述


The SpinPrep PCR Clean-Up Kit is designedfor the rapid purification of DNA amplifiedin PCR. The 10-minute procedure involvesaddition of a binding buffer followed byadsorption of the DNA to a silica membrane ina spin column format. Following a wash step,the DNA is eluted in low-salt buffer. This kitremoves DNA polymerases, dNTPs, salts, and> 99% of primers so that they do not interferewith downstream applications such as cloning,sequencing, or labeling. PCR products from100 bp to > 12,000 bp can be cleaned up, withrecoveries of amplified DNA in the range of60-90% under standard conditions. Column binding capacity: up to 6 g PCR volume: 100 l/rxn Typical recovery: 6090% Size range: 100 bp to > 12,000 bp Time required:< 10 min


警告


Toxicity: Multiple Toxicity Values, refer to MSDS (O)


组分


•82 mlSpinPrep Bind Buffer •27 mlSpinPrep Wash Buffer •10 mlSpinPrep Elute Buffer •100SpinPrep Filters •100Receiver Tubes •100Eluate Receiver Tubes