产品简称

one vial

NACRES

NA.81

生物来源

human male colorectal tissue (Source disease: adenocarcinoma)

抗体应用

drug transporter assay: suitable;permeability assay: suitable;ADME/TOX

免疫原基因信息

human ... ABCB1(5243), ABCG2(9429)

应用

The following posters and articles demonstrate how Caco-2 cells can be used for cell based assays: Transporter Function in Caco-2 Cells with Targeted P-Glycoprotein, MRP2 and BCRP Gene Knockout Using Zinc Finger Nucleases Comparison of Function and Relative Transporter Protein Concentrations in Caco-2 Cells with Single and Double Knockouts of the ABCB1, ABCG2, and ABCC2 Genes Caco-2 Transporter Knockout Cell Based Assays

一般描述

The C2BBe1 cells, a subclone of Caco-2 cells, correspond to ATCC CRL-2102. The MDR1 and BCRP knockout C2BBe1 cells are adenocarcinoma, epithelial cells from a human caucasian male (aged 72 years) with functional knockout of the ABCB1 and ABCG2 (MDR1/BCRP) efflux transporters.

特点和优势

The Caco-2 subclone C2BBe1 cells are ideal for transporter analysis as they express multiple transporters, are human derived, and grow in a homogenous monolayer that forms tight junctions necessary for efflux ratio analysis. Other benefits include: •A functional double knockout of the MDR1 gene and BCRP gene eliminates the reliance on chemical inhibitors to determine if a compound is an MRP2 substrate •The vial format enables the MDR1 and BCRP knockout cells to be included in standard drug transporter protocols •Human assay with no interference from animal inhibitors •Overcome the limitations of RNAi and knockdown cell lines that arise from remaining transporter functionality

法律信息

ADME/Tox Cell Lines License