生物来源

human female skin (Source Disease: Malignant melanoma)

抗体应用

cell culture | mammalian: suitable

应用

This product is a human A375 melanoma cell line in which a landing pad cassette has been integrated into the AAVS1 safe harbor locus using CompoZr® Zinc Finger Nuclease technology. The mKATE2 fluorescence gene was integrated following the EF1a promoter and flanked by unique Cre-lox sites. The design of this landing pad cassette allows for easy, fast, and affordable genetic modification using Cre recombinase. mKATE2 can easily be exchanged for a payload of the user’s choice using Cre recombinase and a targeting vector with appropriate lox sites. Cells can then be sorted via fluorescence-activated cell sorting (FACS) for loss of mKATE2 expression as a surrogate for successful integration of the targeting vector. Approximately 7-10 days are required for loss of the mKATE2 signal in successfully targeted cells. See technical bulletin for detailed protocols.

一般描述

The STR profile of this cell line matches that of its parental cell line ATCC® Catalog No. CRL-1619. A375 are a human malignant melanoma cell line isolated from a 54 year old female. The cells possess a hypotriploid karyotype.

特点和优势

These cells contain the mKATE2 fluorescence gene flanked by unique Cre-lox sites inserted in the AAVS1 safe harbor gene. These A375 cells are adherent with a doubling time of approximately 22 hours.

质量

Tested for Mycoplasma, bacterial and fungal content, post-freeze viability, short terminal repeat (STR) analysis for cell line identification.

培养基

DMEM (Catalog Number D6429) with 10% FBS (Catalog Number F2442)

细胞系描述

These cells are a human malignant melanoma cell line isolated from a 54-year old female. The cells possess a hypotriploid karyotype.