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1 vial
NA.81
human female liver (Source Disease: Hepatocarcinoma and Hepatitis C)
human ... ABCB1(5243)
See technical bulletin for detailed protocols
HepaRG is a human hepatoma cell line. The cells possess a pseudodiploid karyotype and have been characterized as an oval ductular bipotent hepatic cell line as they have the ability to differentiate into both biliary and hepatocyte lineages in the presence of DMSO. HepaRG MDR1 knockout cells express the major xenobiotic sensors (PXR, CAR and AhR), drug transporters, phase I and II drug metabolizing enzymes as well as key hepatic transcription factors involved in stress response pathways.
Zinc finger nucleases (ZFN) mediated Knock-out of MDR1 gene. •The frame-shift mutation of MDR1 gene was confirmed by fragment length analysis and DNA sequencing. •Loss of functionality was confirmed by loss of transport of selective substrates in sandwich culture assay.
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, cytochrome oxidase I (COI) analysis for cell line species confirmation
These products are covered by the License Agreement as described in Exhibit 1 and 2, in the technical bulletin.
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