产品简称

sufficient for 10 preparations

NACRES

NA.52

应用

On-Column DNase I Digestion Set is suitable for removing gDNA from total RNA preparations. It may not be suitable for RT-PCR applications as a small amount of residual DNA may remain. On-Column DNase I Digestion Set was used to purify RNA preparations isolated from Vitis vinifera cultivars.

一般描述

通过下文命名的 Sigma 试剂盒标准方案中提供的程序，将绝大多数 DNA 从总 RNA 纯化中去除。然而，对于非常灵敏的应用，如定量 RT-PCR，即使微量的 DNA 也会导致 cDNA 来源扩增子的假阳性检测。 柱上 DNase I 消化套装为从总 RNA 制备物中去除基因组 DNA 提供了一种简单方便的方法。本套装拟与 GenElute ™ Mammalian Total RNA Miniprep Kit、GenElute Bacterial Total RNA Purification Kit、GenElute Yeast Total RNA Purification Kit 以及 Spectrum™ Plant Total RNA Kit配套使用。不过酶切方案与许多其他市售的 RNA 纯化试剂盒兼容。 术语“柱上”表示消化是在过程中完成的，以确保方便。Sigma的每个总RNA纯化试剂盒都使用结合柱进行纯化。 DNase I消化在15分钟内完成，同时总RNA与柱底物结合。 随后立即进行几次洗涤步骤和总RNA的洗脱。 要查看完整的方案，请参阅上面列出的任何工具包的技术公告。

特点和优势

The On-Column DNase I Digestion Set offers a simple and convenient method for eliminating genomic DNA from total RNA preparations. This Set is intended for use with the GenElute™ Mammalian Total RNA Miniprep Kit, the GenElute Bacterial Total RNA Purification Kit, the GenElute Yeast Total RNA Purification Kit and the Spectrum™ Plant Total RNA Kit. However, the digestion protocol is compatible with many other commercially available RNA purification kits.

原理

The on-column digestion is accomplished in-process for convenience. DNase I digestion is accomplished in 15 minutes while total RNA is bound to the column substrate. Several wash steps and elution of the total RNA immediately follow.