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Heat-labile, recombinant, expressed in Pichia pastoris, Triton-free
expressed in Pichia pastoris
The high salt tolerance and easy removal makes this SAN useful in protein purification schemes, especially in combination with IMAC (Immobilized metal affinity chromatography) media used for purification of poly-His-tagged proteins.
Temp: 5 – 40°C, optimal: ~ 35°C Salt concentration (NaCI / KCI): 150 - 900 mM, optimal: 400 - 650 mM Mg2+: >1 mM is required for activity, optimal: 5 - 50 mM pH: 7.3 – 10, optimal: 8.2 - 9.2 Not recommended to use in presence of SDS or urea.
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