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Type V, lyophilized powder, 250-750 units/mg protein
NA.54
含 Tris 缓冲盐的冻干粉
适用于通过 Williamson, D. H., and Mellanby, J., Methods of Enzymatic Analysis, Bergmeyer, H., ed., 2nd edition, 4, 1836 (1974) 中介绍方法的测定乙酰乙酸酯和 ᴅ(-)-3-羟基丁酸酯。
含 Tris 缓冲盐的冻干粉
In mammalian systems, β-hydroxybutyrate dehydrogenase is localized on the inner mitochondrial membrane and requires phosphatidyl choline for activity. In contrast, the enzyme from Rhodopseudomonas is a soluble cytosolic enzyme that does not require a phospholipid allosteric activator. The enzyme is required for the utilization of ketone bodies as a source of metabolic energy. It catalyzes the oxidation of 3-hydroxybutyrate to acetoacetate, the first step in the conversion of ketone bodies to citric acid, which is then further metabolized via the tricarboxylic acid cycle (Krebs cycle).
One unit will oxidize 1.0 μmole of ᴅ-β-hydroxybutyrate to acetoacetate per min at pH 7.8 at 37 °C.
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