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DeepBio得分:5567.2
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recombinant, expressed in E. coli, ≥90% (SDS-PAGE)
NA.32
human
expressed in E. coli
human ... PTPN2(5771)
P17706
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Protein tyrosine phosphatase, Catalytic domain, (amino acids 2-315), (Gene bank accession number BC016727) with N-terminal GST tag, MW = 62.3 kDa, expressed in an E. coli expression system.
Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.
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