产品简称

recombinant, expressed in HEK 293 cells

产品线

ZooMAb® learn more

抗体产品类型

primary antibodies

物种反应性

human

生物来源

rabbit

单抗克隆号

1I8, recombinant monoclonal

抗体分形

IgG

是否重组表达式

expressed in HEK 293 cells

偶连

unconjugated

抗体应用

affinity binding assay: suitable;immunocytochemistry: suitable;immunohistochemistry: suitable;inhibition assay: suitable;western blot: suitable

免疫原蛋白ID

NP_001243019

免疫原基因信息

human ... ATF2(1386)

增强验证

recombinant expressionLearn more about Antibody Enhanced Validation

免疫原蛋白记录号

P15336

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

应用

Quality Control Testing Evaluated by Western Blotting in lysate from serum starved and EGF treated HepG2 cells. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ATF-2 phosphorylated on Thr 71 in lysate from overnight serum starved HepG2 cells treated with recombinant human EGF (200 ng/mL; 30 min), but not in lysates from untreated cells. Tested applications Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected ATF-2 phosphorylated on Threonine 71 in lysate from HeLa cells treated with Anisomycin (25 g/mL; 30 min). Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected ATF-2 phosphorylated on Threonine 71 in HepG2 cells treated with EGF (200 ng/mL; 30 min). Affinity Binding Assay: A representative lot of this antibody bound Phospho-ATF-2-Thr71 peptide with a KD of 1.6 x 10-8 in an affinity binding assay. Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected ATF-2 phosphorylated on Threonine 71 in human prostate cancer tissue sections. Peptide Inhibition Assay: Target band detection in HepG2 cell lysate was prevented by preblocking of a representative lot with the immunogen phosphopeptide, but not the corresponding non-phosphopeptide. Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user Evaluated by Western Blotting in lysate from serum starved and EGF treated HepG2 cells. Western Blotting Analysis (WB): A 1:1,000 dilution of this antibody detected ATF-2 phosphorylated on Thr 71 in lysate from overnight serum starved HepG2 cells treated with recombinant human EGF (200 ng/mL; 30 min), but not in lysates from untreated cells.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

一般描述

ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity,