产品简称

affinity isolated antibody, buffered aqueous glycerol solution

NACRES

NA.44

抗体产品类型

primary antibodies

物种反应性

mouse, rat, human

生物来源

rabbit

单抗克隆号

polyclonal

偶连

unconjugated

抗体应用

immunoprecipitation (IP): 1:250;western blot (chemiluminescent): 1:1,000

免疫原基因信息

human ... RPS6KB1(6198)mouse ... Rps6kb1(72508)rat ... Rps6kb1(83840)

免疫原蛋白记录号

P23443

外形

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

应用

Anti-phospho-S6-kinase (p70S6K) may be used for immunoblotting at a working dilution of 1:1000 in serum-treated NIH-3T3 cells. For immunoprecipitation, the recommended dilultion is 1:250.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

一般描述

S6 kinase (p70s6k) is activated by an upstream kinase, mTOR in response to nutrients and growth factors. S6 kinase (p70s6k) is a critical regulatory component and mTOR along with the eukaryotic initiation factor 4E (eIF4E)–binding protein 1 (4E-BP1) and S6 kinase (p70s6k) leads to increased ribosomal biogenesis and hence translation. As a critical component and has the ability to modify the cellular responses to availability of nutrients, and growth factors like insulin and IGF-1 when mTOR is chronically active. In presence of excess and chronic insulin, or high fat diets, S6 kinase (p70s6k) regulates by a negative feedback loop and constitutively shuts down the responsiveness of cells to insulin. Thus, mTOR through the S6 kinase (p70s6k) plays a pivotal role in the development of insulin resistance and type 2 diabetes. mTOR signaling via p70S6 kinase also drives cell growth and proliferation, cancer and aging. Anti-phospho-S6-kinase (p70S6K) (phosphothreonine 421/phosphoserine424) detects p70 S6 kinase and p85 S6 kinase only when activated by phosphorylation at Thr421/Ser424 and does not cross-react with other phosphoylated protein kinases.

外形

Solution in 10 mM sodium HEPES, pH 7.5, containing 150 mM sodium chloride, 100 μg/ml bovine serum albumin and 50% glycerol

免疫原

synthetic double phospho-Thr⁴²¹/Ser⁴²⁴ peptide corresponding to residues around Thr⁴²¹/Ser⁴²⁴ of human p70S6K.

特异性

Does not react with non-phosphorylated S6 kinase (p70S6K) or other phosphorylated protein kinases.