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liquid, clone JLA20, Calbiochem®
primary antibodies
mouse
JLA20, monoclonal
IgM
human ... ACTB(60)
100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
Immunoblotting (1:5000-1:20000) Immunofluorescence (1:10000, see application references) Recomended dilution for secondary antibody (0.05 µg/ml, 1:2000 dilution)
Actin is found in all animal cells. Actin monomers have a molecular weight of ~42 kDa. For best results, use the anti-mouse IgM HRP conjugate supplied with the anti-actin antibody. Useful for normalizing protein loading. Antibodies should be titrated for optimal results in individual systems.
Recognizes the ~42 kDa α-, β-, and γ isoforms of actin. Useful as a positive control antibody.Antibody Target Gene Symbol: ACTA1, ACTB1, ACTG1Target Synonym: AA959943, ACTA, Acta-2, Acts, Actsk-1, ALPHA SKELETAL ACTIN, ALPHA1 ACTIN, ASMA, CFTD, CFTD1, CFTDM, Fetal isoform of skeletal alpha-actin, G-actin, MPFD, NEM1, NEM2, NEM3, SKA, SKELETAL ALPHA-ACTIN, SKELETAL MUSCLE ACTINEntrez Gene Name: actin, alpha 1, skeletal muscleHu Entrez ID: 58, 71 (Related Antibodies: CP64)Mu Entrez ID: 11459 ;-; 11465Rat Entrez ID: 29437 ;-; 287876
100 µl ascites diluted in 5 mM sodium phosphate buffer, pH 7.5 and 5 µg anti-mouse IgM, HRP conjugate supplied at 100 µg/ml in 0.05 M sodium phosphate, 50% glycerol.
chicken cytoskeletal proteins
Positive Control All animal cell lines
Following initial thaw, aliquot ascites and freeze (-20°C).
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
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