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DeepBio得分:5567.2
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产品名称：CCK4 Mouse mAb
规格或纯度：ExactAb™, Validated, Carrier Free, 1.0 mg/mL
应用：WB, IP
特异性：CCK4
种属反应性：人(Human)
免疫原：Recombinant protein of human CCK4 (AA 41-210).
阳性对照：WB: Hep G2, HeLa, Jurkat, A431 and HEK293 cell lysates. IP: HCT116 lysates.
偶联：Unconjugated
克隆类型：单克隆抗体
克隆号：K40054_12F7
Format：Whole IgG
亚型：Mouse IgG1
轻链亚型：kappa
SDS-PAGE：150 kDa
纯化方法：Protein A purified
物理外观：Liquid
储存缓冲液：150mM PBS, 0.09% Sodium azide, pH 7.4.
防腐剂：0.09% Sodium azide
浓度：1.0 mg/mL
储存温度：-80℃储存,避免反复冻融
运输条件：特低温运输
稳定性与储存：在 2-8 ℃ 下保存（3 个月）。在 -20 ℃ 下保存（12 个月）。在 -80 ℃ 下保存（36 个月）。收货后建议分装。避免冷冻/解冻循环。
宿主种属：小鼠(Mouse)
种属反应性：人(Human)
克隆号：K40054_12F7
偶联：Unconjugated
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CCK4 Mouse mAb (Ab093426) - Western Blot
All lanes: CCK4 Mouse mAb (Ab093426) at 1/1000 dilution
Samples: Lysates at 20 µg per lane
Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab179001) at 1/20000 dilution
Predicted band size: 118 kDa
Observed band size: 70, 155 kDa
Exposure time: 104.7 s
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CCK4 Mouse mAb (Ab093426) - IP
Immunoprecipitation was performed by incubation of 2.5 µg of CCK4 Mouse mAb (Ab093426) with HCT116 lysate containing 200 µg of total protein. After absorption with Protein G beads, the mixture was run on 6-18% SDS-PAGE and blotted onto nitrocellulose membrane. CCK4 Mouse mAb (Ab093426) at 1 µg/mL was used as the primary antibody and peroxidase conjugated goat anti-mouse IgG was used as the secondary antibody. Lane 1: HCT116 lysateLane 2: PTK7 immunoprecipitated from HCT116 lysate by CCK4 Mouse mAb (Ab093426)
Lane 3: The isotype control antibody instead of CCK4 Mouse mAb (Ab093426) in HCT116 lysate. Result: CCK4 Mouse mAb (Ab093426) can immunoprecipitate PTK7.
查看阿拉丁官网此产品相关对应页面：https://www.aladdin-e.com/zh_cn/Ab093426.html
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