Description

Get maximum activity and stability in proteomic applications with Thermo Scientific™ Pierce™ LysN Protease, MS Grade, a highly purified native Lys-N protease.

Thermo Scientific Pierce LysN Protease, MS Grade, is highly purified native Lys-N protease that has been validated for maximum activity and stability in proteomic applications.

• Thermostable – provides better digestion at higher temperatures (e.g., 50°C) in less time
• Complementary to tryptic digests – cleaves the opposite side of lysine from trypsin
• High purity – no additional (off-target) protease activity detected
• N-terminal lysine cleavage specificity – at least 90% for a complex protein sample
• Versatile – enzyme is effective at many temperatures and in denaturing conditions
• Stable – provided in a lyophilized format

• Improved sequence coverage of protein digests
• De novo sequencing
• Epigenetic studies
• In-solution digestion of proteins

LysN protease is active over a wide range of temperatures and denaturing conditions. Efficient protein digestion can be completed in 2 hours at 50°C or 4 hours at 37°C, however, digestion is possible at both lower and higher temperatures. LysN also remains active under moderate denaturing conditions including 0.1% SDS, 6M urea or heating to 70°C. Maximal LysN activity occurs at pH 7 to 9. The lyophilized enzyme is stable for 2 years when stored at -20°C, and reconstituted stock solutions of LysN are stable at -80°C for 2 years or -20°C for one year without significant loss in activity.