Anti-Aggrecan antibody [RM2036]

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Anti-Aggrecan antibody [RM2036]

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货号：ab315486

品牌：abcam/艾博抗

计量单位：支

交货周期

：7

选择规格： 1mL 20uL 100uL 40uL

运杂费：登录后可查看运杂费

服务电话(移)：17319309550

DeepBio得分:5567.2

DeepBio得分是基于文献引用次数，影响因子，文献新近度等因素计算的客观产品评级，得分越高表明该产品经过越可靠的实验验证，质量可信度越高

产品详情

产品属性

退换货说明：不支持退换货	产地：进口	别名： Primary Antibodies
性状：固体	存储条件： -20°C	保质期： 1年

产品名称

Anti-Aggrecan抗体[RM2036]
参阅全部 Aggrecan 一抗
描述

兔重组multiclonal [RM2036] to Aggrecan
宿主

Rabbit
经测试应用

适用于: IP, WB, IHC-Pmore details
不适用于: Flow Cyt (Intra) or ICC/IF
种属反应性

与反应: Mouse, Rat, Human
免疫原

This product was produced with the following immunogens:
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Human synovial fluid, Mouse articular cartilage, Rat articular cartilage, Human articular cartilage and 293T transfected with a Human ACAN expression vector containing a His-tag, cell lysates. IHC-P: Human cartilage, Mouse cartilage, Rat cartilage and HEK-293T transfected with a ACAN expression vector containing a his tag. IP: Mouse articular cartilage lysate.
常规说明
This product is a recombinant multiclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA
Concentration information loading...
纯度

Protein A purified
克隆

Recombinant Multiclonal
克隆编号

RM2036
同种型

IgG
研究领域

Alternative Versions
- Anti-Aggrecan antibody [RM2036] - BSA and Azide free (ab315487)
Compatible Secondaries
- VeriBlot for IP Detection Reagent (HRP) (ab131366)
- Goat Anti-Rabbit IgG H&L (HRP) (ab97051)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Related Products
- Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602)
- Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204)

The Abpromise guarantee

Abpromise™承诺保证使用ab315486于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
IP		1/30.
WB		1/1000. Predicted molecular weight: 250 kDa.
IHC-P		1/2000 - 1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

说明
IP 1/30.
WB 1/1000. Predicted molecular weight: 250 kDa.
IHC-P 1/2000 - 1/10000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

应用说明

Is unsuitable for Flow Cyt (Intra) or ICC/IF.

相关性

Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS.
细胞定位

Secreted; extracellular matrix.
数据库链接
- Entrez Gene: 176 Human
- Entrez Gene: 11595 Mouse
- Entrez Gene: 58968 Rat
- Omim: 155760 Human
- SwissProt: P16112 Human
- SwissProt: Q61282 Mouse
- SwissProt: P07897 Rat
别名
- ACAN antibody
- AGC 1 antibody
- AGC1 antibody
- AGCAN antibody
- Aggrecan 1 (chondroitin sulfate proteoglycan 1, large aggregating proteoglycan, antigen identified by monoclonal antibody A0122) antibody
- Aggrecan 1 antibody
- Aggrecan core protein antibody
- Aggrecan proteoglycan antibody
- Aggrecan structural proteoglycan of cartilage antibody
- Aggrecan1 antibody
- ATEGQV antibody
- Cartilage specific proteoglycan core protein antibody
- Chondroitin sulfate proteoglycan 1 antibody
- Chondroitin sulfate proteoglycan 1 large aggregating proteoglycan antigen identified by monoclonal antibody A0122 antibody
- Chondroitin sulfate proteoglycan core protein 1 antibody
- CSPG 1 antibody
- CSPG1 antibody
- CSPGCP antibody
- JSCATE antibody
- Large aggregating proteoglycan antibody
- mcspg antibody
- mgsk16 antibody
- MSK 16 antibody
- MSK16 antibody
- SEDK antibody
see all

Western blot - Anti-Aggrecan antibody [RM2036] (ab315486)

All lanes : Anti-Aggrecan antibody [RM2036] (ab315486) at 1/1000 dilution

Lane 1 : Human synovial fluid lysate
Lane 2 : Human liver tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 250 kDa
Observed band size: 50-350 kDa why is the actual band size different from the predicted?

Exposure time: 180 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: liver.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11425904, 16188468).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-Aggrecan antibody [RM2036] (ab315486)

All lanes : Anti-Aggrecan antibody [RM2036] (ab315486) at 1/1000 dilution

Lane 1 : Mouse articar cartilage tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Mouse liver tissue lysate
Lane 4 : Rat articar cartilage tissue lysate
Lane 5 : Rat spleen tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 250 kDa
Observed band size: 50-350 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

Negative control: liver, spleen.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11425904, 16188468).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

Exposure time: Lanes 1-3: 1second, lanes 4-5: 3 seconds.
Western blot - Anti-Aggrecan antibody [RM2036] (ab315486)

Anti-Aggrecan antibody [RM2036] (ab315486) at 1/1000 dilution + Human articar cartilage tissue lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Predicted band size: 250 kDa
Observed band size: 50-350 kDa why is the actual band size different from the predicted?

Exposure time: 8 seconds

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID: 11425904, 16188468).

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.
Western blot - Anti-Aggrecan antibody [RM2036] (ab315486)

All lanes : Anti-Aggrecan antibody [RM2036] (ab315486) at 1/1000 dilution

Lane 1 : 293T cells transfected with an empty vector containing a His-tag, whole cell lysate
Lane 2 : 293T cells transfected with a Human ACAN expression vector containing a His-tag, whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 250 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?

Exposure time: 1 second

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) staining at 1/200000 dilution.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) staining at 1/5000 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [RM2036] (ab315486)

Immunohistochemical analysis of paraffin-embedded Human cartilage tissue labeling Aggrecan with ab315486 at 1/4000 (0.132 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on human cartilage. The section was incubated with ab315486 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [RM2036] (ab315486)

Immunohistochemical analysis of paraffin-embedded Mouse cartilage tissue labeling Aggrecan with ab315486 at 1/4000 (0.132 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on mouse cartilage. The section was incubated with ab315486 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [RM2036] (ab315486)

Immunohistochemical analysis of paraffin-embedded Rat cartilage tissue labeling Aggrecan with ab315486 at 1/10000 (0.053 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on rat cartilage. The section was incubated with ab315486 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [RM2036] (ab315486)

Immunohistochemical analysis of paraffin-embedded (A) HEK-293T (human embryonic kidney epithelial cell) transfected with a ACAN expression vector containing a his tag. (B) HEK-293T transfected with empty vector containing a his tag. tissue labeling Aggrecan with ab315486 at 1/2000 (0.263 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Positive staining on (A) HEK-293T transfected with a ACAN expression vector containing a his tag, no staining on (B) HEK-293T transfected with empty vector containing a his tag. The section was incubated with ab315486 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Aggrecan antibody [RM2036] (ab315486)

Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Aggrecan with ab315486 at 1/4000 (0.132 ug/ml) dilution, followed by a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Negative control: no staining on human liver. The section was incubated with ab315486 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Secondary antibody only control: Secondary antibody is a ready to use LeicaDS9800 (Bond™ Polymer Refine Detection).

Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, Epitope Retrieval Solution2) for 20 mins