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性状 ： 固体 |
存储条件 ： ﹣20° |
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保质期 ： 1年 |
Anti-F4/80 antibody (ab6640) recognises the mouse F4/80 antigen, a 160kD glycoprotein expressed by murine macrophages.
Tissue, cells or virus corresponding to F4/80. Thioglycollate stimulated peritoneal macrophages from C57/BL mice
Please note we cannot guarantee IHC-P, however should you use this application for this product then please use a specific protocol which can be found in the protocols section of our datasheet here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Concentration information loading...| 应用 | Ab评论 | 说明 |
|---|---|---|
| Flow Cyt |
1/10 - 1/50.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
| ICC/IF | (11) |
Use a concentration of 5 - 10 µg/ml.
We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
| 说明 |
|---|
|
Flow Cyt
1/10 - 1/50. ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
|
ICC/IF
Use a concentration of 5 - 10 µg/ml. We recommend ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) secondary antibody. |
Target information above from: UniProt accession
Q14246
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-266714-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg)
ab6640 stained Raw 264.7 cells. The cells were 100% methanol fixed for 5 minutes at room temperatureand then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6640 at 5µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was ab150165 Goat Anti-Rat IgG H&L (Alexa Fluor® 488) preadsorbed used at a 1/1000 dilution for 1 hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-264399-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/10 followed by streptavidin FITC secondary at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
![Immunocytochemistry/ Immunofluorescence - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-12433-anti-f4-80-antibody-cia3-1-immunofluorescence.jpg)
![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-264401-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow cytometry analysis of peritoneal macrophages labelling F4/80 (red) with ab6640 at a dilution of 1/10. The blue line shows J774 cells stained with Rat anti mouse isotype control.
![Flow Cytometry - Anti-F4/80 antibody [CI:A3-1] - Macrophage Marker (ab6640)](https://www.abcam.cn/ps/products/6/ab6640/Images/ab6640-264400-anti-f4-80-antibody-cia3-1-flow-cytometry.jpg)
Flow cytometry analysis of J774 cells labelling F4/80 (red) with ab6640 at a dilution of 1/50 followed by goat anti rat IgG FITC secondary antibody at 1/100 dilution. The blue line shows J774 cells stained with Rat anti mouse isotype control.
发表研究结果有使用 ab6640？请让我们知道，以便我们可以引用本数据表中的参考文章。
ab6640 被引用在 1093 文献中.
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