Goat Anti-Mouse IgG H&L (HRP)

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Goat Anti-Mouse IgG H&L (HRP)

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货号：ab6789

品牌：abcam/艾博抗

计量单位：支

交货周期

：7

选择规格： 1mg

运杂费：登录后可查看运杂费

服务电话(移)：17319309550

DeepBio得分:5567.2

DeepBio得分是基于文献引用次数，影响因子，文献新近度等因素计算的客观产品评级，得分越高表明该产品经过越可靠的实验验证，质量可信度越高

产品详情

产品属性

退换货说明：不支持退换货	性状：固体	存储条件： ﹣20°
保质期： 1年

产品名称

山羊抗小鼠IgG H&L (HRP)
参阅全部 IgG 二抗
宿主

Goat
靶标种属

Mouse
经测试应用

适用于: ICC, IP, Dot blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WBmore details
免疫原

mouse IgG whole molecule
偶联物

HRP

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C. Store In the Dark.
存储溶液

Preservative: 0.01% Gentamicin sulphate
Constituents: 1% BSA, 0.424% Potassium phosphate solution, 0.88% Sodium chloride
Concentration information loading...
纯度

Affinity purified
纯化说明

MOUSE IgG (H&L) Antibody Peroxidase Conjugated was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
共轭说明

Horseradish Peroxidase (HRP)
克隆

多克隆
同种型

IgG
常规说明

The product can contain small precipitates giving a cloudy appearance. However this does not affect the performace of the product. Leaving the product at room temperature in the dark for a few hours may help. Please use as normal and if you do experience problems, contact Scientific Support.
研究领域
- Secondary antibodies
- anti-Mouse
- IgG
- Enzyme
- HRP

Related Products
- Normal Goat Serum (ab7481)
Substrate reagent

The Abpromise guarantee

Abpromise™承诺保证使用ab6789于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC		1/500 - 1/2500.
IP		Use at an assay dependent concentration. PubMed: 17164758
Dot blot		Use at an assay dependent concentration.
ELISA		1/100000.
IHC-P		1/500 - 1/2500.
IHC-Fr		1/500 - 1/2500.
Immunomicroscopy		Use at an assay dependent concentration.
WB	(3)	1/2000 - 1/10000.

说明
ICC 1/500 - 1/2500.
IP Use at an assay dependent concentration. PubMed: 17164758
Dot blot Use at an assay dependent concentration.
ELISA 1/100000.
IHC-P 1/500 - 1/2500.
IHC-Fr 1/500 - 1/2500.
Immunomicroscopy Use at an assay dependent concentration.
WB 1/2000 - 1/10000.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

IHC image of beta actin staining in human colon formalin fixed paraffin embedded tissue section*.

The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH 6) for 30 mins. The section was incubated with ab8224, 3 µg/ml overnight at +4°C. An HRP-conjugated secondary (ab6789, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with hematoxylin and mounted with DPX.

The inset negative control image is secondary-only at 1/500 dilution.

*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

All lanes : Anti-CDC42 Binding Protein Kinase Beta antibody (ab61328) at 1/2000 dilution

All lanes : Cytosolic fraction prepared from murine brain tissue

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Mouse IgG H&L (HRP) (ab6789) at 1/2000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Observed band size: 194 kDa why is the actual band size different from the predicted?

Exposure time: 1 minute
Immunoprecipitation - Goat Anti-Mouse IgG H&L (HRP) (ab6789)This image is courtesy of an anonymous Abreview.

ab49969 Immunoprecipitating HA Tag in HEK-293T whole cell lysate. 25 µg of cell lysate was incubated with primary antibody (1/2500 in lysis buffer) and matrix (Protein A/G) for 6 hours at 4°C. For western blotting a HRP-conjugated goat anti-mouse IgG H&L (ab6789) (1/1000) was used to confirm successful immunoprecipation.
See Abreview
Western blot - Goat Anti-Mouse IgG H&L (HRP) (ab6789)

ab6789 was used at dilution 1/10000 with the primary antibody ab55504 in WB. See the review on ab55504.

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheet download

Download

发表研究结果有使用 ab6789？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab6789 被引用在 1584 文献中.

Fan M et al. IRE1α regulates the PTHrP-IHH feedback loop to orchestrate chondrocyte hypertrophy and cartilage mineralization. Genes Dis 11:464-478 (2024). PubMed: 37588212
Bou T et al. Muscle fibre transition and transcriptional changes of horse skeletal muscles during traditional Mongolian endurance training. Equine Vet J 56:178-192 (2024). PubMed: 37345447
Zhang XR et al. Effect of Silicone Patch Containing Metal-organic Framework on Hypertrophic Scar Suppression. In Vivo 38:235-245 (2024). PubMed: 38148076
Chen J et al. MiRNA-26a-5p inhibits preterm labor initiation by targeting and regulating TRPC3 ion channel protein expression. Environ Toxicol 39:357-366 (2024). PubMed: 37755144
Ping N et al. Translational enhancement of target endogenous mRNA in mammalian cells using programmable RNA-binding pentatricopeptide repeat proteins. Sci Rep 14:251 (2024). PubMed: 38167853

View all Publications for this product

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Goat Anti-Mouse IgG H&L (HRP)

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概述

产品名称

宿主

靶标种属

经测试应用

免疫原

偶联物

性能

形式

存放说明

存储溶液

纯度

纯化说明

共轭说明

克隆

同种型

常规说明

研究领域

相关产品

Related Products

Substrate reagent

应用

The Abpromise guarantee

图片

实验方案

数据表及文件

Datasheet download

文献 (1584)