Anti-alpha smooth muscle Actin antibody [1A4]

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Anti-alpha smooth muscle Actin antibody [1A4]

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货号：ab7817

品牌：abcam/艾博抗

计量单位：支

交货周期

：7

选择规格： 1mg 100ug

运杂费：登录后可查看运杂费

服务电话(移)：17319309550

DeepBio得分:5567.2

DeepBio得分是基于文献引用次数，影响因子，文献新近度等因素计算的客观产品评级，得分越高表明该产品经过越可靠的实验验证，质量可信度越高

产品详情

产品属性

退换货说明：不支持退换货	性状：固体	存储条件： ﹣20°
保质期： 1年

产品名称

Anti-alpha smooth muscle Actin抗体[1A4]
参阅全部 alpha smooth muscle Actin 一抗
描述

小鼠单克隆抗体[1A4] to alpha smooth muscle Actin
宿主

Mouse
经测试应用

适用于: ICC/IF, Flow Cyt (Intra), IHC-P, WBmore details
种属反应性

与反应: Mouse, Rat, Human
预测可用于: Sheep, Rabbit, Cow, Pig, Mammals, Baboon
免疫原

Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).
Database link: P62736
阳性对照
- WB: Human foreskin fibroblast lysate, human colon tissue lysate; NIH/3T3, SV40LT-SMC whole cell lysates. Flow Cyt (Intra): SV40LT-SMC. IHC-P: Human breast ductal carcinoma tissue. ICC/IF: SV40LT-SMC cells.
常规说明

This antibody clone [1A4] is manufactured by Abcam.

If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
存储溶液

pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine
Concentration information loading...
纯度

Protein G purified
克隆

单克隆
克隆编号

1A4
同种型

IgG2a
轻链类型

kappa
研究领域

Alternative Versions
Compatible Secondaries
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
- Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) preadsorbed (ab150117)
Isotype control
- Mouse IgG2a, kappa monoclonal [MG2a-53] - Isotype control (ab18415)
KO cell lines
- Human ACTA2 knockout HeLa cell line (ab264014)
KO cell lysates
- Human ACTA2 knockout HeLa cell lysate (ab264499)
Positive Controls
- Jurkat whole cell lysate (ab7899)
Recombinant Protein
- Recombinant Human alpha smooth muscle Actin protein (ab134555)
Related Products

The Abpromise guarantee

Abpromise™承诺保证使用ab7817于以下的经测试应用

“应用说明”部分下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

应用	Ab评论	说明
ICC/IF	(22)	Use a concentration of 1 µg/ml.
Flow Cyt (Intra)		Use a concentration of 1.137 µg/ml.
IHC-P	(40)	Use a concentration of 0.034 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB	(15)	Use a concentration of 1 µg/ml.

说明
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt (Intra) Use a concentration of 1.137 µg/ml.
IHC-P Use a concentration of 0.034 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use a concentration of 1 µg/ml.

功能

Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
疾病相关

Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
序列相似性

Belongs to the actin family.
细胞定位

Cytoplasm > cytoskeleton.
Target information above from: UniProt accession P62736 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 101021287 Baboon
- Entrez Gene: 515610 Cow
- Entrez Gene: 59 Human
- Entrez Gene: 11475 Mouse
- Entrez Gene: 733615 Pig
- Entrez Gene: 100009271 Rabbit
- Entrez Gene: 81633 Rat
- Entrez Gene: 101121051 Sheep
- Omim: 102620 Human
- SwissProt: P62739 Cow
- SwissProt: P62736 Human
- SwissProt: P62737 Mouse
- SwissProt: P62740 Rabbit
- SwissProt: P62738 Rat
- Unigene: 500483 Human
- Unigene: 213025 Mouse
- Unigene: 195319 Rat
- Unigene: 3114 Rat
see all
别名
- a actin antibody
- AAT6 antibody
- ACTA_HUMAN antibody
- ACTA2 antibody
- Actin alpha 2 smooth muscle aorta antibody
- Actin aortic smooth muscle antibody
- Actin, aortic smooth muscle antibody
- ACTSA antibody
- ACTVS antibody
- Alpha 2 actin antibody
- Alpha actin 2 antibody
- Alpha cardiac actin antibody
- alpha sma antibody
- Alpha-actin-2 antibody
- Cell growth inhibiting gene 46 protein antibody
- Cell growth-inhibiting gene 46 protein antibody
- GIG46 antibody
- Growth inhibiting gene 46 antibody
- MYMY5 antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

Immunohistochemical analysis of formalin fixed paraffin embedded human colon labelling alpha smooth muscle actin with ab7817 at a concentration of 0.01µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 mins at 100°C with ULTRA cell conditioning solution (CC1, pH 8.5). ab7817 anti-alpha smooth muscle actin antibody [1A4] was incubated at 37°C for 16 mins. Sections were counterstained with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)This image is courtesy of an Abreview submitted by David Ivancic

Immunohistochemistry analysis (Formalin/PFA-fixed paraffin-embedded sections) of 10% NBF-fixed human colon tissue permeabilized with 0.05% tween 20. Stained with ab7817 at 1/100 dilution. Secondary antibody used was Alexa fluor® 488 Donkey anti-Rabbit IgG at 1/300 dilution. Blocking was done with Sea Block for 30 minutes at 22°C. The sample was incubated with the primary antibody and Sea Block for 14 hours at 4°C. Antigen retrieval method was heat mediated, ab94674 100X Citrate Buffer pH 6.0.
See Abreview
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

ab7817 staining alpha smooth muscle Actin in SV40LT-SMC cells (positive control, top panel) and A431 cells (negative control, bottom panel). The cells were fixed with 100% methanol (5 min) then permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated with ab7817 at 1μg/ml concentration and ab6046 (Rabbit polyclonal to beta Tubulin) at 1/1000 dilution overnight at 4°C followed by a further incubation at room temperature for 1h with a goat secondary antibody to mouse IgG (Alexa Fluor^® 488) (ab150117) at 2 μg/ml (shown in green) and a goat secondary antibody to rabbit IgG (Alexa Fluor^® 594) (ab150080) at 2 μg/ml (shown in red). Nuclear DNA was labelled in blue with DAPI.
Image was taken with a confocal microscope (Leica-Microsystems TCS SP8).
Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1 µg/ml

Lane 1 : NIH 3T3 whole cell lysate
Lane 2 : SV40LT-SMC whole cell lysate
Lane 3 : A431 whole cell lysate
Lane 4 : A549 whole cell lysate
Lane 5 : Jurkat whole cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800RD) at 1/10000 dilution

Observed band size: 42 kDa why is the actual band size different from the predicted?

Gel type: MOPS

Blocking buffer: 3% milk

Loading control: alpha tubulin (ab52866), secondary Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (1:10000 dilution)
Western blot - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

All lanes : Anti-alpha smooth muscle Actin antibody [1A4] (ab7817) at 1 µg/ml

Lane 1 : Human colon tissue lysate
Lane 2 : Mouse colon tissue lysate
Lane 3 : Human Foreskin Fibroblast Whole Cell Lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Mouse IgG H&L (IRDye® 800RD) at 1/10000 dilution

Observed band size: 42 kDa why is the actual band size different from the predicted?

Gel type: MOPS

Blocking buffer: 3% milk

Loading control: alpha tubulin (ab52866), secondary Goat anti-Rabbit IgG H&L (IRDye® 680CW) preadsorbed (1:10000 dilution)
Immunocytochemistry/ Immunofluorescence - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

This data was developed using the same antibody clone in a different buffer formulation that is PBS and sodium azide free (ab240654)

ab240654 staining alpha smooth muscle Actin in SV40LT-SMC cells. The cells were fixed with 4% paraformaldehyde (10 min), permeabilized with 0.1% PBS-Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab240654 at 1µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor^® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor^® 594) at 1/1000 dilution (shown in pseudocolour red). Nuclear DNA was labelled with DAPI (shown in blue).

Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [1A4] (ab7817)

IHC image of alpha smooth muscle actin staining in a human breast ductal carcinoma formalin fixed paraffin embedded tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solut