WB: Human and rat duodenum lysate. HeLa and NIH/3T3 whole cell lysate.
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
形式
Liquid
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
A 1 hour primary incubation at room temperature is recommended for this product.
说明
WB Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42 kDa).
A 1 hour primary incubation at room temperature is recommended for this product.
靶标
功能
Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
疾病相关
Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6) [MIM:611788]. AATs are characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. They are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance.
Hongo H et al.Somatic GJA4 gain-of-function mutation in orbital cavernous venous malformations.Angiogenesis 26:37-52 (2023).
PubMed: 35902510
Zeng W et al.CCL18 signaling from tumor-associated macrophages activates fibroblasts to adopt a chemoresistance-inducing phenotype.Oncogene 42:224-237 (2023).
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Dudakovic A et al.The epigenetic regulator BRD4 is required for myofibroblast differentiation of knee fibroblasts.J Cell Biochem 124:320-334 (2023).
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Zhang R et al.Rate of castration-induced prostate stroma regression is reduced in a mouse model of benign prostatic hyperplasia.Am J Clin Exp Urol 11:12-26 (2023).
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Patzek S et al.Loss of Fgf9 in mice leads to pancreatic hypoplasia and asplenia.iScience 26:106500 (2023).
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